HEPES Buffer CAS #7365-45-9
HEPES buffer(4 ?C (2-hydroxyethyl) ?C 1-piperazine ethanesulfonic acid) is a zwitterionic sulfonic acid buffer; One of good??s 20 buffers. Hepes buffer is widely used in cell culture, mainly because it can maintain physiological pH value better than bicarbonate buffer, which is also commonly used in cell culture, although the concentration of carbon dioxide (produced by aerobic respiration) changes. Lepe Zuniga et al. An unwanted photochemical process in which hepes buffer produces hydrogen peroxide when exposed to ambient light is reported, which is not a problem in bicarbonate based cell culture buffer. Therefore, it is strongly recommended to place the solution containing hepes buffer in the dark as far as possible to prevent oxidation.
HEPES Buffer CAS #7365-45-9
| HEPES Basic information |
| Product Name: | HEPES |
| Synonyms: | HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%;N-(2-HYDROXYETHYL)-PIPERAZINE-N’-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID));Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz;1-HeptaneSulfonicAcidSod.SaltGr;N-(2-Hydroxyethyl)Piperazine-N’-(3-EthanesulfonicAcid);N-(2-Hydroxyethyl)Piperazine-N’-(2-EthanesulfonicAcid)(Hepes);Hepes,LowSodium,FreeAcid |
| CAS: | 7365-45-9 |
| MF: | C8H18N2O4S |
| MW: | 238.3 |
| EINECS: | 230-907-9 |
| Product Categories: | Biochemistry;Good’s Buffers;Buffer;ReagentsBiological Buffers;AlphabeticalMolecular Biology;Biological Buffers;BioUltra Buffers;BioUltraMolecular Biology;DNA&RNA Purification;Molecular Biology Reagents;Zwitterionic;Disinfectants;GMP;7365-45-9 |
| Mol File: | 7365-45-9.mol |
 |
|
| HEPES Chemical Properties |
| Melting point | 234-238 ??C |
| Boiling point | 408??[at 101 325 Pa] |
| density | 1.07?g/mL?at 20???C |
| vapor pressure | 0Pa at 25?? |
| refractive index | n20/D?1.339 |
| storage temp. | 2-8??C |
| solubility | H2O: 1?M at?20???C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| pka | 7.5(at 25??) |
| PH Range | 7.0 – 7.6 |
| Water Solubility | Soluble |
| ??max | ??: 260 nm Amax: 0.06 ??: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| LogP | -3.85 at 20?? |
| CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
Related Products
Chemical Name: UV-120
Other Name: (2’,4’-Di-tert-butylphenyl 3,5-di-tert-butyl-4-hydroxybenzoate)
CAS No.: 4221-80-1
Molecular Fomula: C29H42O3
Molecular weight: 438.66
Assay: ≥99%（LC）
Chemical Name: Azidotrimethylsilane
CAS No.: 4648-54-8
Appearance:?Transparent Liquid
Assay???? 95%
Chloramine-T is an N-terminally chlorinated and N-terminally deprotonated sulfonamide used as an insecticide and mild disinfectant.
Prismatic crystal, soluble in water, almost insoluble in benzene, chloroform and ether. Decay in ethanol. There is no certain melting point, as well as it is violently decayed (exploded) at 175-180 ??. With minor chlorine scent, it progressively sheds water in dry air, and slowly decomposes in exposed air to launch chlorine.
Chloramine-T can be prepared in a return of 75?C 95% by presenting chlorine into the salt hydroxide option of p-toluenesulfonamide. It is a solid electrolyte in acid remedy and a great oxidant in alkali option. Conveniently soluble in water, nearly insoluble in benzene, chloroform and ether. The substance can conveniently react with mustard gas to generate safe crystalline thioimides; Chloramine-T derivatives are being studied as poison gas guards.
Chemical Name: o-Xylene
Synonyms: 1,2-Dimethylbenzene; ortho-xylene
CAS No.: 95-47-6
Molecular Formula: C8H10
Molecular Weight: 106.17
Chemical Name: 3-Hydroxybutyric acid
CAS No.: 625-71-8
Molecular Formula: C4H8O3
Molecular Weight: 104.1
Appearance: White powder
Chemical Name: N-(Trimethylsilyl)imidazole
CAS No.:18156-74-6
Molecular Formula: C6H12N2Si
Molecular weights: 140.26
Appearance: colorless to yellow liquid
Content: ??98%
Chemical Name: Potassium Castorate
CAS No.: 8013-05-6
Molecular Formula: C57H107K3O12
Molecular Weight: 1101.74718
Appearance: Yellow Liquid
Chemical Name: STODDARD SOLVENT
CAS No.: 64742-88-7
Appearance: Colorless or Light Yellow Liquid
Chemical Name: TMB (monosulfate)
CAS No.:54827-18-8
Molecular weight:338.42
Appearance:White to off-white solid
Chemical name:DFHBI 1T
CAS No.: 1539318-36-9
Molecular Fomula: C13H9F5N2O2
Molecular weight:?320.21
Appearance: white crystal
Assay: ??98.0%
AMBERLITE XAD-16 is a nonionic, hydrophobic, cross-linked polymeric adsorbent supplied as insoluble white beads. It is characterized by its macroreticular structure, which provides both a continuous polymer phase and a continuous pore phase, offering high surface area and porosity . This adsorbent is specifically designed to capture hydrophobic molecules from polar solvents and volatile organic compounds from vapor streams . It is ideal for use in column or batch operations and is effective for the adsorption of organic substances with relatively low to medium molecular weight . The product is widely utilized in applications such as the purification of antibiotics, recovery of water-soluble steroids, amino acids, and proteins, as well as the removal of nonpolar compounds from polar solvents .
Chemical Name: Quercetin-3-O-sophoroside
CAS No.: 18609-17-1
Molecular Formula: C27H30O17
Molecular Weight: 626.52
