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Fluorescein is a synthetic organic compound that appears as a dark orange/red powder and is slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.

Fluorescein is a fluorescent group commonly used in microscopes, dye lasers as gain media, forensic and serological detection of potential blood stains, and dye tracing. The maximum absorption wavelength of fluorescein is 494 nm, and the maximum emission wavelength is 521 nm (in water). The main derivatives are fluorescein isothiocyanate (FITC) and 6-FAM phosphorous amide in oligonucleotide synthesis.