HEPES Buffer CAS #7365-45-9
HEPES buffer(4 ?C (2-hydroxyethyl) ?C 1-piperazine ethanesulfonic acid) is a zwitterionic sulfonic acid buffer; One of good??s 20 buffers. Hepes buffer is widely used in cell culture, mainly because it can maintain physiological pH value better than bicarbonate buffer, which is also commonly used in cell culture, although the concentration of carbon dioxide (produced by aerobic respiration) changes. Lepe Zuniga et al. An unwanted photochemical process in which hepes buffer produces hydrogen peroxide when exposed to ambient light is reported, which is not a problem in bicarbonate based cell culture buffer. Therefore, it is strongly recommended to place the solution containing hepes buffer in the dark as far as possible to prevent oxidation.
HEPES Buffer CAS #7365-45-9
| HEPES Basic information |
| Product Name: | HEPES |
| Synonyms: | HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%;N-(2-HYDROXYETHYL)-PIPERAZINE-N’-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID));Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz;1-HeptaneSulfonicAcidSod.SaltGr;N-(2-Hydroxyethyl)Piperazine-N’-(3-EthanesulfonicAcid);N-(2-Hydroxyethyl)Piperazine-N’-(2-EthanesulfonicAcid)(Hepes);Hepes,LowSodium,FreeAcid |
| CAS: | 7365-45-9 |
| MF: | C8H18N2O4S |
| MW: | 238.3 |
| EINECS: | 230-907-9 |
| Product Categories: | Biochemistry;Good’s Buffers;Buffer;ReagentsBiological Buffers;AlphabeticalMolecular Biology;Biological Buffers;BioUltra Buffers;BioUltraMolecular Biology;DNA&RNA Purification;Molecular Biology Reagents;Zwitterionic;Disinfectants;GMP;7365-45-9 |
| Mol File: | 7365-45-9.mol |
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| HEPES Chemical Properties |
| Melting point | 234-238 ??C |
| Boiling point | 408??[at 101 325 Pa] |
| density | 1.07?g/mL?at 20???C |
| vapor pressure | 0Pa at 25?? |
| refractive index | n20/D?1.339 |
| storage temp. | 2-8??C |
| solubility | H2O: 1?M at?20???C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| pka | 7.5(at 25??) |
| PH Range | 7.0 – 7.6 |
| Water Solubility | Soluble |
| ??max | ??: 260 nm Amax: 0.06 ??: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| LogP | -3.85 at 20?? |
| CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
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